Analysis of VCP Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2038-LGZ)

Name: Analysis of VCP Gene (Mutation) by RT-qPCR
SKU: STEM-MT-2038-LGZ
Rating: 5 (1 reviews)

Introduction

Official Full Name: valosin containing protein
Also known as: p97; TERA; CDC48; FTDALS6
This gene encodes a member of the AAA atpase protein family. The encoded protein plays a role in protein degradation, cell membrane fusion, DNA repair and replication, cell cycle regulation, activation of NF-κB pathway, etc. The protein forms a homohexameric complex, interacts with various cofactors, and extracts ubiquitinated proteins from lipid membranes or protein complexes. In human patients, mutations in this gene cause IBMPFD (inclusion body myopathy with paget disease of bone and frontotemporal dementia), ALS (amyotrophic lateral sclerosis) and Fibula-Marie-dental disease.

Buy Now

Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.