Analysis of UBE2T Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0741-LGZ)

Name: Analysis of UBE2T Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0741-LGZ
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Introduction

Official Full Name: ubiquitin conjugating enzyme E2 T
Also known as: FANCT; PIG50; HSPC150
The protein encoded by this gene catalyzes the covalent attachment of ubiquitin to protein substrates. Defects in this gene have been associated with Fanconi anemia of complementation group T. Two transcript variants encoding different isoforms have been found for this gene.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.