Analysis of TRAF3IP1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1665-LGZ)

Name: Analysis of TRAF3IP1 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1665-LGZ
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Introduction

Official Full Name: TRAF3 interacting protein 1
Also known as: IFT54; MIPT3; SLSN9; FAP116; MIP-T3; CFAP116
The protein encoded by this gene interacts with TNF receptor-associated factor 3, tethering it to cytoskeletal microtubules. The encoded protein is also an inhibitor of the innate type I IFN response. Defects in this gene are a cause of Senior-Loken syndrome 9.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.