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Analysis of TPRG1L Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1441-LGZ)
Introduction
Official Full Name: tumor protein p63 regulated 1 like
Also known as: TPRGL; mover; FAM79A; SVAP30; h-mover
Predicted to enable identical protein binding activity. Predicted to be involved in regulation of glutamatergic synaptic transmission. Located in extracellular exosome.
Principle
Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.
Applications
Gene mutation analysis.
Procedure
1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.
Materials
Sample: depends on the customer's analysis requirements
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For various laboratory needs
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