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Analysis of Tooth Structure by Raman Spectroscopy (CAT#: STEM-ST-0100-WXH)
Introduction
Tooth enamel is the most mineralized tissue of human body. The composition is 96 wt% inorganic material and 4 wt% organic material and water. In dentin, the inorganic material represents 70 wt%. This inorganic material is mainly composed of calcium phosphate related to the hexagonal hydroxyapatite, whose chemical formula is [Ca10(PO4)6(OH)2].
Principle
Raman Spectroscopy is a non-destructive chemical analysis technique which provides detailed information about chemical structure, phase and polymorphy, crystallinity and molecular interactions.
The principle behind Raman spectroscopy is that the monochromatic radiation is passed through the sample such that the radiation may get reflected, absorbed, or scattered. The scattered photons have a different frequency from the incident photon as the vibration and rotational property vary.
The principle behind Raman spectroscopy is that the monochromatic radiation is passed through the sample such that the radiation may get reflected, absorbed, or scattered. The scattered photons have a different frequency from the incident photon as the vibration and rotational property vary.
Applications
• Analysis of biocompatibility of a material.
• Analysis of nucleic acids.
• Study of interactions between drugs and cells.
• Photodynamic therapy (PDT).
• Analyzing metabolic accumulations of a substance or compounds.
• Diagnosis of disease.
• Analysis of individual cells.
• Cell sorting applications.
• Analyzing the features of biomolecules.
• Study of bone structure.
• Analysis of nucleic acids.
• Study of interactions between drugs and cells.
• Photodynamic therapy (PDT).
• Analyzing metabolic accumulations of a substance or compounds.
• Diagnosis of disease.
• Analysis of individual cells.
• Cell sorting applications.
• Analyzing the features of biomolecules.
• Study of bone structure.
Procedure
1. Preparation of samples
2. Determine instrument parameters
3. Perform background scan
4. Test the sample
5. Data analysis
2. Determine instrument parameters
3. Perform background scan
4. Test the sample
5. Data analysis
Materials
• Raman Spectrometer
• Raman Imaging Microscope
• Raman Imaging Microscope
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For various laboratory needs
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