Analysis of TNFRSF18 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1230-LGZ)

Name: Analysis of TNFRSF18 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1230-LGZ
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Introduction

Official Full Name: TNF receptor superfamily member 18
Also known as: AITR; GITR; CD357; GITR-D; ENERGEN
This gene encodes a member of the TNF receptor superfamily. The encoded receptor has been shown to increase expression upon T cell activation and is thought to play a key role in the maintenance of dominant immune self-tolerance by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest a role for this receptor in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants encoding different isoforms of this gene have been reported.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.