Analysis of TNF-α (Mouse) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0595-LGZ)

Introduction

Tumor necrosis factor α (TNF-α) is a potent paracrine and endocrine mediator of inflammation and immune function. It is also thought to regulate the growth and differentiation of various cell types. TNF-α is selectively cytotoxic to many transformed cells, especially when used in combination with IFNα.

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Principle

Detection Technique: MSD Hypersensitive Multi-Factor Electrochemiluminescence
Capture Antibody: Hamster Monoclonal
Detection Antibody: Goat Polyclonal
Recombinant Standards: Expressed in E. coli

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma, Urine, Cell Culture Supernatant
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