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Analysis of TGF-α (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0571-LGZ)

Introduction

Gene expression of transforming growth factor alpha (TGF-α) is upregulated in some human cancers and is associated with tumor metastasis and invasion. Produced in macrophages, brain cells and keratinocytes, it induces epithelial development and has a similar effect to the receptor for EGF (Epithelial Growth Factor). TGFα stimulates the proliferation of nerve cells in the damaged adult brain.

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Principle Applications Procedure Materials

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Growth Factors, Cardiovascular

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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