Analysis of SMYD3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0313-LGZ)

Name: Analysis of SMYD3 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0313-LGZ
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Introduction

Official Full Name: SET and MYND domain containing 3
Also known as: KMT3E; ZMYND1; ZNFN3A1; bA74P14.1
This gene encodes a histone methyltransferase which functions in RNA polymerase II complexes by an interaction with a specific RNA helicase. Multiple transcript variants encoding different isoforms have been found for this gene.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.