Analysis of SLAMF8 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0654-LGZ)

Name: Analysis of SLAMF8 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0654-LGZ
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Introduction

Official Full Name: SLAM family member 8
Also known as: BLAME; CD353; SBBI42
This gene encodes a member of the CD2 family of cell surface proteins involved in lymphocyte activation. These proteins are characterized by an Ig domain. The protein is expressed in lymphoid tissues, and studies of similar proteins in mice suggest that it may play a role in B-cell lineage commitment. This gene is located in a region of chromosome 1 that contains many CD2 genes.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.