Analysis of SigD-Regulated Genes in Bacillus Subtilis by Northern Blot Technology (CAT#: STEM-MHT-0131-LGZ)

Introduction

Northern blot was invented in 1977 by James Alwine, David Kemp and George Stark at Stanford University. Because its basic principle and operation process are very similar to Southern blot, it is called Northern blot, which is mainly used to analyze the expression of gene mRNA. Although methods for detecting gene expression include QPCR, gene chips, and RNase protection experiments, Northern blot has better sensitivity than gene chips; compared with QPCR, it has higher specificity and can effectively reduce false positives in experimental results. Northern blot experiments are still considered an accurate method for detecting gene expression.<br />The soil Gram-positive bacterium Bacillus subtilis requires flexibility as to dramatic changes in external and internal conditions at the gene expression level to survive. The B. subtilis genome contains 18 sigma factors including Xpf Helmann and Moran. SigD RNA polymerase is an alternative sigma factor, and is present and active in the late exponential growth phase.