Analysis of SDHAF2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2455-LGZ)

Name: Analysis of SDHAF2 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-2455-LGZ
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Introduction

Official Full Name: succinate dehydrogenase complex assembly factor 2
Also known as: PGL2; SDH5; hSDH5; C11orf79
This gene encodes a mitochondrial assembly factor needed for the flavination of a succinate dehydrogenase complex subunit (SDHA), which is required for activity of the succinate dehydrogenase complex. Mutations in this gene are associated with paraganglioma.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.