Analysis of RNF115 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0614-LGZ)

Name: Analysis of RNF115 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0614-LGZ
Rating: 5 (1 reviews)

Introduction

Official Full Name: ring finger protein 115
Also known as: BCA2; ZFP364; ZNF364; RABRING7
Enables ubiquitin-protein transferase activity. Involved in negative regulation of epidermal growth factor receptor signaling pathway; protein autoubiquitination; and ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway. Predicted to be located in cytosol.

Buy Now

Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.