Analysis of RAB3GAP1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1474-LGZ)

Name: Analysis of RAB3GAP1 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1474-LGZ
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Introduction

Official Full Name: RAB3 GTPase activating protein catalytic subunit 1
Also known as: P130; MARTS2; WARBM1; RAB3GAP; RAB3GAP130
This gene encodes the catalytic subunit of a Rab GTPase activating protein. The encoded protein forms a heterodimer with a non-catalytic subunit to specifically regulate the activity of members of the Rab3 subfamily of small G proteins. This protein mediates the hydrolysis of GTP bound Rab3 to the GDP bound form. Mutations in this gene are associated with Warburg micro syndrome. Alternate splicing results in multiple transcript variants.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.