Analysis of protein dynamics by Quench-Flow method (CAT#: STEM-AC-0042-WXH)

Name: Analysis of protein dynamics by Quench-Flow method
SKU: STEM-AC-0042-WXH
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Introduction

Protein dynamics refers to the study of the movement and conformational changes of proteins over time. This field of research is crucial for understanding protein function, as the dynamic nature of proteins enables them to interact with other molecules, undergo conformational changes, and perform their biological functions.

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Principle Applications Procedure Materials

Principle

The principle is to mix the two solutions and then to observe a change in spectroscopic properties of the mixture at different place along the reaction tube during the flow. In the continuous mode of the quenched-flow method, the observation chamber is replaced by a second mixer in which the quenching agent arrives.

Applications

Used to determine fast reaction rates or single turnover rates of enzymatic reactions and to isolate reaction intermediates.

Procedure

Small volumes of solutions are driven through a high efficiency mixer and flow into a delay (or ageing) loop. After a set time, the reaction is stopped (or quenched) by the addition of a chemical quench solution.