Analysis of POGZ Gene (Mutation) in Inborn Gene (Mutation)tic Diseases by RT-qPCR (CAT#: STEM-MT-0140-LGZ)

Name: Analysis of POGZ Gene (Mutation) in Inborn Gene (Mutation)tic Diseases by RT-qPCR
SKU: STEM-MT-0140-LGZ
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Introduction

Also known as: MRD37; WHSUS; ZNF635; ZNF280E; ZNF635m
The protein encoded by this gene appears to be a zinc finger protein containing a transposase domain at the C-terminus. This protein was found to interact with the transcription factor SP1 in a yeast two-hybrid system. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Inborn genetic diseases

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.