Analysis of PIGM Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0574-LGZ)

Name: Analysis of PIGM Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0574-LGZ
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Introduction

Official Full Name: phosphatidylinositol glycan anchor biosynthesis class M
Also known as: GPI-MT-I
This gene encodes a transmembrane protein located in the endoplasmic reticulum and involved in GPI anchor biosynthesis. The glycosylphosphatidylinositol (GPI) anchor is a glycolipid with three mannose molecules in its core backbone. GPI anchors are present in many blood cells and serve to anchor proteins to the cell surface. This gene encodes a mannosyltransferase, GPI-MT-I, that transfers the first mannose to GPI on the lumenal side of the endoplasmic reticulum.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.