Analysis of phosphorylated peptides by ion mobility-mass spectrometry (CAT#: STEM-ST-0123-LJX)

Name: Analysis of phosphorylated peptides by ion mobility-mass spectrometry
SKU: STEM-ST-0123-LJX
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Introduction

Phosphorylated polypeptides mainly refer to the modification of the side chain hydroxyl groups of Ser, Tyr and Thr residues in the peptide chain into acid phosphate polypeptides. Phosphorylated peptides are indispensable tools to study the phosphorylation process of proteins, so it is very important to study the phosphorylation reaction of proteins and peptides and determine the mature and simple synthesis route. Phosphoropeptides in protein digestives can be quickly screened by ion migration mass spectrometry.

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Principle Applications Procedure Materials Notes

Principle

Ion mobility spectrometry–mass spectrometry (IMS-MS) is an analytical chemistry method that separates gas phase ions based on their interaction with a collision gas and their masses. In the first step, the ions are separated according to their mobility through a buffer gas on a millisecond timescale using an ion mobility spectrometer. The separated ions are then introduced into a mass analyzer in a second step where their mass-to-charge ratios can be determined on a microsecond timescale.

Applications

For studying the gas phase ion structure
For detecting the chemical warfare agents and explosives
For the analysis of proteins, peptides, drug-like molecules and nano particles
For monitoring isomeric reaction intermediates and probe their kinetics
For proteomics and pharmaceutical analysis

Procedure

1. Add sample
2. The ions in the sample are separated in the ion mobility spectrometer
3. The separated ions are introduced into the mass analyzer for detection
4. Store the detection results

Materials

• Sample Type:
Phosphorylated peptides

Notes

1. Ion mobility spectrometry is also a very fast technique, making it suitable for high-throughput applications. The entire analysis can be completed in just a few minutes.
2. The method is extremely sensitive and able to detect trace amounts of contaminants that other spectrometry methods would miss.
3. The effective separation of analytes achieved with this method makes it widely applicable in the analysis of complex samples such as in proteomics and metabolomics.

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