Analysis of Phage capsid by Differential Scanning Fluorimetry(DSF) (CAT#: STEM-MB-0833-WXH)

Name: Analysis of Phage capsid by Differential Scanning Fluorimetry(DSF)
SKU: STEM-MB-0833-WXH
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Introduction

Capsids are highly ordered proteinaceous structures utilized by phages to protect their genomes. Phages with the most frequently identified capsid shapes, filamentous and icosahedral, have been modified using genetic and chemical methods to functionalize phages with an array of organic and inorganic materials.

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Principle Applications Procedure Materials

Principle

Differential Scanning Fluorimetry measures protein thermal unfolding by monitoring changes in fluorescence emission of a sample upon heating. This allows the determination of protein thermostability and complex formation even with weakly binding ligands by thermal shift assay. Differential Scanning Fluorimetry is therefore ideally suited for screening of optimum buffer conditions like pH, buffer composition and ionic strength. The technique is applicable to any biological sample, from soluble proteins to integral membrane proteins.

Applications

To identify low-molecular-weight ligands that bind and stabilize purified proteins.
To measure the denaturation and unfolding of proteins.

Procedure

1. Preparation of compound solutions
2. Preparation of buffer/additive screen plates
3. Preparation of compound storage plates
4. Equipment preparation
5. Sample preparation
87. Performing the scan