Analysis of P-Cadherin (Human) by ELISA (CAT#: STEM-MB-1116-LGZ)

Name: Analysis of P-Cadherin (Human) by ELISA
SKU: STEM-MB-1116-LGZ
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Introduction

Placental(P)-Cadherin(PCAD) belongs to the family of adhesion molecules and is a calcium-dependent transmembrane protein. Couples with catenins, α, β, and γ (plakoglobin) in the cytoplasm, and then associates with the cytoskeleton. Plays an important role in embryonic formation, especially tissue formation. It is also involved in maintaining the structure of mature tissues. Human PCAD has 829 amino acids in synthesis and 722 amino acids in mature protein.

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Principle Applications Procedure Materials

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Intracellular Signaling

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.