Analysis of OPTC Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0383-LGZ)

Name: Analysis of OPTC Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0383-LGZ
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Introduction

Official Full Name: opticin
Also known as: OPT
Opticin belongs to class III of the small leucine-leucine-rich repeat protein (SLRP) family. Members of this family are often associated with the extracellular matrix. Opsins are abundant in the vitreous of the eye, but also in the cornea, iris, ciliary body, optic nerve, choroid, retina, and fetal liver. Opticin can non-covalently bind collagen fibrils and regulate the morphology, spacing and organization of fibrils. The opticin gene was mapped to a region of chromosome 1 associated with the inherited eye diseases age-related macular degeneration (AMD) and ataxia of the posterior column of retinitis pigmentosa (AXPC1).

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.