Analysis of MPZL1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0477-LGZ)

Name: Analysis of MPZL1 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0477-LGZ
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Introduction

Official Full Name: myelin protein zero like 1
Also known as: PZR; PZRa; PZRb; PZR1b; MPZL1b
Predicted to enable structural molecule activity. Predicted to be involved in cell-cell signaling and transmembrane receptor protein tyrosine kinase signaling pathway. Predicted to act upstream of or within positive regulation of cell migration. Located in cell surface and focal adhesion.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.