Analysis of MICB by ELISA (CAT#: STEM-MB-1537-LGZ)

Name: Analysis of MICB by ELISA
SKU: STEM-MB-1537-LGZ
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Introduction

As a ligand of human NKG2D type II receptor, MICB (Human Histocompatibility Complex Class I associated gene B) is a transmembrane glycoprotein. The MICB gene is located in the MHC-I region of human chromosome 6 and is polymorphic, with more than 15 known human alleles. Micbs are expressed only in trace amounts in normal cells. They are usually expressed in epithelial tumors and can be induced by bacterial and viral infections. Therefore, the identification of MICBs is closely related to tumor surveillance, viral infection and the prevention of autoimmune diseases.

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Principle Applications Procedure Materials

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.