Analysis of METTL18 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0409-LGZ)

Name: Analysis of METTL18 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0409-LGZ
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Introduction

Official Full Name: methyltransferase 18, RPL3 N3(tau)-histidine
Also known as: HPM1; AsTP2; C1orf156
Enables heat shock protein binding activity and protein-L-histidine N-tele-methyltransferase activity. Involved in peptidyl-lysine monomethylation; regulation of gene expression; and regulation of ribosome biogenesis. Located in cytosol and nucleolus. Part of protein-containing complex.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.