Analysis of MAP4K3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1732-LGZ)

Name: Analysis of MAP4K3 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1732-LGZ
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Introduction

Official Full Name: mitogen-activated protein kinase kinase kinase kinase 3
Also known as: GLK; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1
This gene encodes a member of the mitogen-activated protein kinase kinase kinase kinase family. The encoded protein activates key effectors in cell signalling, among them c-Jun. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.