Analysis of LPGAT1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0437-LGZ)

Name: Analysis of LPGAT1 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0437-LGZ
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Introduction

Official Full Name: lysophosphatidylglycerol acyltransferase 1
Also known as: NET8; FAM34A; LPLAT7; FAM34A1
This gene encodes a member of the lysophospholipid acyltransferase family. The encoded protein catalyzes the reacylation of lysophosphatidylglycerol to phosphatidylglycerol, a membrane phospholipid that is an important precursor for the synthesis of cardiolipin. Alternative splicing results in multiple transcript variants.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.