Analysis of JNK (Human, Mouse, Rat) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0377-LGZ)

Introduction

JNK, (c-Jun N-terminal kinase), also known as stress-activated protein kinase (SAPK), is another subclass of MAPK (mitogen-activated protein kinase) signaling pathway in mammalian cells. It plays an important role in various physiological and pathological processes such as cell cycle, reproduction, apoptosis and cell stress.

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Principle

Detection Technique: MSD Hypersensitive Multi-Factor Electrochemiluminescence
Capture Antibody: Rabbit Polyclonal
Detection Antibody: Mouse Monoclonal

Applications

Phosphoproteins, Intracellular Signaling

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Whole Cell Lysate
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