Analysis of IL24 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1068-LGZ)

Name: Analysis of IL24 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1068-LGZ
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Introduction

Official Full Name: interleukin 24
Also known as: C49A; FISP; MDA7; MOB5; ST16; IL10B
This gene encodes a member of the IL10 family of cytokines. It was identified as a gene induced during terminal differentiation of melanoma cells. The protein encoded by this gene can selectively induce apoptosis of various tumor cells. Overexpression of this gene leads to increased expression of multiple genes in the GADD family, which is related to the induction of apoptosis. This gene induces phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38) and heat shock protein 27kDa 1 (HSPB2/HSP27) in melanoma cells but not in normal immortalized melanocytes. Alternative splicing transcript variants encoding different isoforms have been reported.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.