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Analysis of IL-37 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0346-LGZ)
Introduction
Interleukin (IL-37) is a new member of the IL-1 family, with five different subtypes (IL-37a-e). Currently, only IL-37b has been shown to have biological functions and anti-inflammatory effects in a variety of diseases. IL-37 can either be secreted outside the cell as a cytokine, bind to receptors to regulate cell activity, or enter the nucleus as a transcription regulator.
Principle
MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.
Applications
Immunology/Inflammation
Procedure
1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.
Materials
• Sample Type: Plasma, Serum
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