Analysis of IL-12p70 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0297-LGZ)

Introduction

Interleukin-12 (IL-12) is a cytokine with a wide range of biological activities, mainly produced by activated inflammatory cells. IL-12 is a heterodimer composed of two chains of p35 and p40 (IL-12β), and the two subunits are linked together by disulfide bonds. This structure is rare in the discovered IL. Typically, the amount of p40 far exceeds that of p70.

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Principle

Detection Technique: MSD Hypersensitive Multi-Factor Electrochemiluminescence
Capture Antibody: Mouse Monoclonal
Detection Antibody: Mouse Monoclonal
Recombinant Standards: Expressed in Insect cell line

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma, Urine, Cell Culture Supernatant
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