Analysis of HAPLN2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1052-LGZ)

Name: Analysis of HAPLN2 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1052-LGZ
Rating: 5 (1 reviews)

Introduction

Official Full Name: hyaluronan and proteoglycan link protein 2
Also known as: BRAL1
Predicted to enable hyaluronic acid binding activity. Predicted to be involved in central nervous system development and skeletal system development. Predicted to act upstream of or within establishment of blood-nerve barrier and extracellular matrix assembly. Predicted to be located in extracellular region. Predicted to be active in extracellular matrix.

Buy Now

Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.