Analysis of H3-3A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1019-LGZ)

Name: Analysis of H3-3A Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1019-LGZ
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Introduction

Official Full Name: H3.3 histone A
Also known as: H3F3; H3-3B; H3.3A; H3F3A; BRYLIB1
Histones are fundamental nuclear proteins responsible for the structure of eukaryotic chromosome fibers in the nucleosome. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, and about 146 bp of DNA are packaged in repeating units called nucleosomes. Linker histone H1 interacts with linker DNA between nucleosomes and plays a role in the compaction of chromatin into higher-order structures Unlike most histone genes, this gene contains introns and its mRNA is polyadenylated. The encoded protein is a replication-independent member of the histone H3 family.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.