Analysis of GITRL/TNFSF18 (Human) by ELISA (CAT#: STEM-MB-0856-LGZ)

Name: Analysis of GITRL/TNFSF18 (Human) by ELISA
SKU: STEM-MB-0856-LGZ
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Introduction

Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also known as GITR or AITR, is a protein encoded by the TNFRSF18 gene. This gene encodes a member of the tumor necrosis factor receptor superfamily. The encoded receptor has been shown to increase expression upon T cell activation and is thought to play a key role in the maintenance of dominant immune self-tolerance by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest a role for this receptor in the regulation of CD3-driven T-cell activation and programmed cell death.

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Principle Applications Procedure Materials

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.