Analysis of FEZ2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1807-LGZ)

Name: Analysis of FEZ2 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1807-LGZ
Rating: 5 (1 reviews)

Introduction

Official Full Name: fasciculation and elongation protein zeta 2
Also known as: HUM3CL
This gene is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles. Other orthologs include the rat gene that encodes zygin II, which can bind to synaptotagmin.

Buy Now

Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.