Analysis of FCGR3B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0955-LGZ)

Name: Analysis of FCGR3B Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0955-LGZ
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Introduction

Official Full Name: Fc gamma receptor IIIb
Also known as: CD16; FCG3; CD16A; CD16b; FCGR3; CD16-I; FCGR3A; FCR-10; FCRIII; FCRIIIb
The protein encoded by this gene is a low-affinity receptor for the Fc region of gamma immunoglobulin (IgG). The encoded protein acts as a monomer and can bind monomeric or aggregated IgG. This gene may have the function of trapping immune complexes in the peripheral circulation. Several transcript variants of this gene have been found encoding different isoforms. A highly similar gene encoding a related protein was also found on chromosome 1.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.