Analysis of FCGR3A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0956-LGZ)

Name: Analysis of FCGR3A Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0956-LGZ
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Introduction

Official Full Name: Fc gamma receptor IIIa
Also known as: CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; CD16-II; FCGRIII; FCRIIIA; FcGRIIIA
This gene encodes the receptor for the Fc portion of immunoglobulin G, which is involved in the removal of antigen-antibody complexes from circulation, as well as other responses, including antibody-dependent cell-mediated cytotoxicity and antibody-dependent enhancement of viral infection. This gene (FCGR3A) is highly similar to another neighboring gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. The Mutations in the gene are associated with immunodeficiency and are associated with susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternative splice transcript variants of this gene have been found to encode distinct isoforms.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.