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Analysis of FCGR1A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0960-LGZ)
Introduction
Official Full Name: Fc gamma receptor Ia
Also known as: CD64; FCG1; FCRI; CD64A; FCGR1; IGFR1; FcgammaRI
This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1.
Principle
Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.
Applications
Gene mutation analysis.
Procedure
1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.
Materials
Sample: depends on the customer's analysis requirements
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For various laboratory needs
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