Analysis of FAM72A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0941-LGZ)

Name: Analysis of FAM72A Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0941-LGZ
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Introduction

Official Full Name: family with sequence similarity 72 member A
Also known as: p17; LMPIP; Ugene
Predicted to act upstream of or within negative regulation of brain-derived neurotrophic factor-activated receptor activity and positive regulation of apoptotic process. Located in cytosol and intracellular membrane-bounded organelle.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.