Analysis of ERCC3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1470-LGZ)

Name: Analysis of ERCC3 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1470-LGZ
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Introduction

Official Full Name: ERCC excision repair 3, TFIIH core complex helicase subunit
Also known as: XPB; BTF2; Ssl2; TTD2; GTF2H; RAD25; TFIIH
This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Xeroderma pigmentosum

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.