Analysis of ENTR1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2037-LGZ)

Name: Analysis of ENTR1 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-2037-LGZ
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Introduction

Official Full Name: endosome associated trafficking regulator 1
Also known as: SDDAG3; NY-CO-3; SDCCAG3
Involved in several processes, including endocytic recycling; positive regulation of cilium assembly; and positive regulation of protein localization to cilium. Located in endosome; microtubule organizing center; and midbody. Colocalizes with retromer complex.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.