Analysis of ENTPD1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2103-LGZ)

Name: Analysis of ENTPD1 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-2103-LGZ
Rating: 5 (1 reviews)

Introduction

Official Full Name: ectonucleoside triphosphate diphosphohydrolase 1
Also known as: CD39; SPG64; ATPDase; NTPDase-1
The protein encoded by this gene is a plasma membrane protein that hydrolyzes extracellular ATP and ADP to AMP. Inhibition of this protein's activity may confer anticancer benefits. Several transcript variants encoding different isoforms have been found for this gene.

Buy Now

Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.