Analysis of ENPP2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1909-LGZ)

Name: Analysis of ENPP2 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1909-LGZ
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Introduction

Official Full Name: ectonucleotide pyrophosphatase/phosphodiesterase 2
Also known as: ATX; NPP2; ATX-X; PDNP2; LysoPLD; AUTOTAXIN; PD-IALPHA
The protein encoded by this gene acts either as a phosphodiesterase, which cleaves the phosphodiester bond at the 5' end of an oligonucleotide, or as a phospholipase, which catalyzes the production of lysophosphatidic acid (LPA) in the extracellular fluid. LPA elicits growth factor-like responses, including stimulation of cell proliferation and chemotaxis. The gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several cancers. Gene products are secreted and further processed to produce biologically active forms. Several alternatively spliced transcript variants encoding different isoforms have been identified.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.