Analysis of Enolase 2 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0145-LGZ)
Introduction
Enolase is an enzyme that catalyzes the formation of the high-energy compound phosphoenolpyruvate from 2-phosphoglycerate, and is one of the key enzymes in glycolysis.
MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.
Applications
Neurobiology/Neurodegeneration, Oncology & Cancer
Procedure
1. Coat the capture antibody on the well plate. 2. Add samples/calibrators. 3. Add detection antibody. 4. Read the plate and analyze the data.