Analysis of ELAPOR1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1188-LGZ)

Name: Analysis of ELAPOR1 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1188-LGZ
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Introduction

Official Full Name: endosome-lysosome associated apoptosis and autophagy regulator 1
Also known as: EIG121; KIAA1324
Expression of this gene is induced by estrogen and the encoded protein has been characterized as a transmembrane protein. The encoded protein has been found in to correlate with survival in certain carcinomas and may be important for cellular response to stress. Alternative splicing results in multiple transcript variants.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.