Analysis of ECM1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0937-LGZ)

Name: Analysis of ECM1 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0937-LGZ
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Introduction

Official Full Name: extracellular matrix protein 1
Also known as: URBWD
This gene encodes a soluble protein involved in endochondral bone formation, angiogenesis and tumor biology. It also interacts with a variety of extracellular and structural proteins to help maintain skin integrity and homeostasis. Mutations in this gene are associated with lipoproteinosis (also known as hyalinosis of the skin and mucous membranes or Urbach-Wiethe disease), which is characterized by widespread thickening of the skin, mucous membranes, and certain internal organs. Alternative splicing transcript variants encoding different isoforms have been described for this gene.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.