Analysis of DYRK3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0938-LGZ)

Name: Analysis of DYRK3 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0938-LGZ
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Introduction

Official Full Name: dual specificity tyrosine phosphorylation regulated kinase 3
Also known as: RED; REDK; DYRK5; hYAK3-2
The gene product belongs to the DYRK family of dual-specificity protein kinases that catalyze the autophosphorylation of serine/threonine and tyrosine residues. Members of this family are structurally similar but differ in substrate specificity, suggesting their involvement in distinct cellular functions. The encoded protein has been shown to autophosphorylate tyrosine residues in vitro and catalyze the phosphorylation of histones H3 and H2B. Alternative splicing transcript variants encoding different isoforms have been identified.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.