Analysis of DSTYK Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0912-LGZ)

Name: Analysis of DSTYK Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0912-LGZ
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Introduction

Official Full Name: dual serine/threonine and tyrosine protein kinase
Also known as: RIP5; RIPK5; SPG23; CAKUT1; RHDNS1; DustyPK; HDCMD38P
This gene encodes a dual serine/threonine and tyrosine protein kinase which is expressed in multiple tissues. It is thought to function as a regulator of cell death. Multiple transcript variants encoding different isoforms have been found for this gene.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.