Analysis of DNAJB2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1516-LGZ)

Name: Analysis of DNAJB2 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1516-LGZ
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Introduction

Official Full Name: DnaJ heat shock protein family (Hsp40) member B2
Also known as: HSJ1; CMT2T; DSMA5; HSJ-1; HSPF3
This gene is almost exclusively expressed in the brain, mainly in the neuronal layers. It encodes a protein that shows sequence similarity to bacterial DnaJ protein and the yeast homologs. In bacteria, this protein is implicated in protein folding and protein complex dissociation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.