Analysis of DNA-ethidiumbromide complex by Linear dichroism (LD) (CAT#: STEM-ST-0133-WXH)

Name: Analysis of DNA-ethidiumbromide complex by Linear dichroism (LD)
SKU: STEM-ST-0133-WXH
Rating: 5 (1 reviews)

Introduction

Ethidium bromide is commonly used as a non-radioactive marker for identifying and visualizing nucleic acid bands in electrophoresis. It fluoresces readily with a reddish-brown color when exposed to ultraviolet. light, intensifying almost 20-fold after binding to DNA.

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Principle

Linear dichroism (LD) or diattenuation is the difference between absorption of light polarized parallel and polarized perpendicular to an orientation axis. It is the property of a material whose transmittance depends on the orientation of linearly polarized light incident upon it. LD measurements are based on the interaction between matter and light and thus are a form of electromagnetic spectroscopy.