Analysis of DIPK1A Gene (Mutation) in Diamond-Blackfan anemia by RT-qPCR (CAT#: STEM-MT-0117-LGZ)

Name: Analysis of DIPK1A Gene (Mutation) in Diamond-Blackfan anemia by RT-qPCR
SKU: STEM-MT-0117-LGZ
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Introduction

Also known as: FAM69A
This gene encodes a member of the FAM69 family of cysteine-rich type II transmembrane proteins. These proteins localize to the endoplasmic reticulum but their specific functions are unknown. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Diamond-Blackfan anemia

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.